The Effect of Alcoholic Essence Satureja Sahandica Bornm L. on the Expression of Metalloproteinases 1, 2, 9 and 13 in a Model Similar to Osteoarthritis

Loss of the extracellular matrix cartilage is one most important and prominent features osteoarthritis (OA). The ECM has two components, including proteoglycan aggrecan type II collagen, which are main targets MMPs ADAM TSs enzymes. In this paper, effect ASSB Compared with dexamethasone ibuprofen as representatives steroidal nonsteroidal anti-inflammatory drugs. One way to prevent irreversible joint damage in OA inhibit MMPs. can improve condition patients by reducing expression 9 13 gene. Background: a progressive disease characterized degeneration, bone regeneration, synovial membrane inflammation exacerbated age. Given side effects conventional treatments, drugs corticosteroids, it consider new treatments for disorder. Recently, metalloproteinases on pathogenesis been attracted attention. Objective: aim paper was evaluate impact Alcoholic Satureja Sahandica Bornem L. (ASSB) essence inhibiting metalloproteinase gene 1, 2, bovine fibroblast-like synoviocytes stimulated lipopolysaccharide model osteoarthritis. Methods: Synovial cells were isolated from articular radiocarpal an 8-month-old Holstein cow. After determining degree toxicity MTT Assay, exposed stimulation LPS without or presence dexamethasone, alcoholic (ASSB). Metalloproteinase measured RT-PCR ELISA. migration cell invasion investigated Transwell chambers. Results: results showed that Bornm (SSB) essential oil significantly reduced 12 synovocytes lipopolysaccharide. It also suppressed these cells. However, had no significant 1 2. Conclusion: Based our results, reduce activity inflammatory OA, its potential role complement NSAIDs common corticosteroids confirmed. cellular modeling does not confirm beneficial patients.